绵羊AA-NAT基因mRNA表达与常年发情相关性研究

doi:10.11843/j.issn.0366-6964.2015.04.005

Abstract

Abstract:

In order to study the association of AA-NAT gene and year-round estrus in sheep，year-round estrous Small Tail Han ewes and seasonal estrous Tan ewes were selected as tested subjects.Real-time PCR method was used to detect AA-NAT mRNA expression in hypothalamus，pituitary gland，pineal gland，ovary，uterus body and adrenal gland at different estrus stages.The results showed that the expression of AA-NAT gene in all 6 tissues at different estrous stages of the 2 breeds was significantly different.The expression of AA-NAT gene was at high level in ovary，uterus body and pineal gland in sheep at estrus.In ovary and uterus body， AA-NAT gene expression was significantly lower (P<0.01) in Small Tail Han sheep at spring and fall estrus stage than that in Tan sheep at fall estrus stage，but in pineal gland it was significantly higher (P<0.01).The results preminarily indicated that the up-regulated expression of AA-NAT gene in ovary，uterus body and pineal gland was associated with year-round estrus in Small Tail Han sheep.

CLC Number:

S826
S814.8

LU Lu-lu，SUN Xiao-di，CHU Ming-xing，WANG Yong-juan，HUANG Dong-wei，WANG Jin-yu，DI Ran，PAN Zhang-yuan，LIU Qiu-yue. Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 542-548.

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References

［1］MIGLIORI M L，ROMANOWSKI A，SIMONETTA S H，et al.Daily variation in melatonin synthesis and arylalkylamine N-acetyltransferase activity in the nematode Caenorhabditis elegans ［J］.J Pineal Res，2012，53(1):38-46.
［2］张凯.牦牛褪黑激素合成酶AA-NAT、HIOMT基因CDS克隆及序列分析［D］.雅安:四川农业大学，2007.
ZHANG K.Molecular cloning and nucleotide sequence analysis of the yak melatonin synthetase genes：AA-NAT and HIOMT ［D］.Ya’an：Sichuan Agricultural University，2007.(in Chinese)
［3］赵瑛.褪黑激素及其合成受体生理功能和病理状态的改变及意义［J］.第二军医大学学报，2007，28(7):755-756.
ZHAO Y.Melatonin and its receptor：physiological function and pathological implication ［J］.Academic Journal of Second Military Medical University，2007，28(7):755-756.(in Chinese)
［4］郭玉强，蔡国宝，刘艳婷，等.褪黑激素对绵羊季节性繁殖的调控机理［J］.甘肃畜牧兽医，2008，38(1):43-46.
GUO Y Q，CAI G B，LIU Y T，et al.Melatonin regulation mechanism of seasonal breeding in sheep［J］.Gansu Animal and Veterinary Sciences，2008，38(1):43-46.(in Chinese)
［5］CASSONE V M.Effects of melatonin on vertebrate circadian systems ［J］.Trends Neurosci，1990，13(11):457-464.
［6］CAVALLO A，RITSCHEL W A.Pharmacokinetics of melatonin in human sexual maturation ［J］.J Clin Endocrinol Metab，1996，81(5):1882-1886.
［7］PUIG-DOMINGO M，WEBB S M，SERRANO J，et al.Melatonin-related hypogonadotropic hypogonadism ［J］.N Engl J Med，1992，327(19):1356-1359.
［8］LINCOLN G A，FRASER H M，FLETCHER T J.Induction of early rutting in male red deer by melatonin and its dependence on LHRH ［J］.J Reprod Fertil，1984，72(2):339-343.
［9］WILSON P R，WALKER I H，BOND D B，et al.Field evaluation of melatonin implants to advance the breeding season in 1-year-old farmed red deer hinds ［J］.N Z Vet J，1991，39(1):23-28.
［10］WEBSTER J R，SUTTIE J M，CORSON I D.Effects of melatonin implants on reproductive seasonality of male red deer ［J］.J Reprod Fertil，1991，92(1):1-11.
［11］张小辉.MLT、LH 和 FSH 对绵羊季节性发情的调控作用研究［D］.杨凌：西北农林科技大学，2004.
ZHANG X H.The regulation of MLT，LH and FSH on the seasonal estrus of sheep［D］.Yangling：Northwest A & F University，2004.(in Chinese)
［12］KIM T D，KIM J S，KIM J H，et al.Rhythmic serotonin N-acetyltransferase mRNA degradation is essential for the maintenance of its circadian oscillation ［J］.Mol Cell Biol，2005，25(8):3232-3246.
［13］陈朝坚，雷申，文勇立，等.藏黄牛褪黑激素合成酶AA-NAT基因cDNA克隆及序列的比较分析［J］.西南民族大学学报:自然科学版，2010，36(2):220-225.
CHEN C J，LEI S，WEN Y L，et al.Molecular cloning and sequence analysis of Tibetan cattle melatonin synthetase AA-NAT gene ［J］.Journal of Southwest University for Nationalities：Natural Science Edition，2010，36(2):220-225.(in Chinese)
［14］ROSEBOOM P H，COON S L，BALER R，et al.Melatonin synthesis：analysis of the more than 150-fold nocturnal increase in serotonin N-acetyltransferase messenger ribonucleic acid in the rat pineal gland ［J］.Endocrinology，1996，137(7):3033-3045.
［15］KLEIN D C，ROSEBOOM P H，COON S L.New light is shining on the melatonin rhythm enzyme：the first postcloning view ［J］.Trends Endocrinol Metab，1996，7(3):106-112.
［16］HARDELAND R，MADRID J A，TAN D X，et al.Melatonin，the circadian multioscillator system and health：the need for detailed analyses of peripheral melatonin signaling ［J］.J Pineal Res， 2012，52(2):139-166.
［17］BARRETT P，BOLBOREA M.Molecular pathways involved in seasonal body weight and reproductive responses governed by melatonin ［J］. J Pineal Res，2012，52(4):376-388.
［18］BITTMAN E L.The role of rhythms in the response to melatonin ［J］.Ciba Found Symp， 1985，117:149-169.
［19］YELLON S M，BITTMAN E L，LEHMAN M N.Importance of duration of nocturnal melatonin secretion in determining the reproductive response to inductive photoperiod in the ewe ［J］.Biol Reprod，1985，32(3):523-529.
［20］KLEIN D C，COON S L，ROSEBOOM P H，et al.The melatonin rhythm-generating enzyme：molecular regulation of serotonin N-acetyltransferase in the pineal gland ［J］.Recent Prog Horm Res，1997，52:307-358.
［21］巩元芳，李祥龙，刘铮铸，等.我国主要地方绵羊品种随机扩增多态DNA研究［J］.遗传，2002，24(4):423-426.
GONG Y F，LI X L，LIU Z Z，et al.Studies of random amplified polymorphic DNA (RAPD) of main indigenous sheep breeds in China［J］.Hereditas，2002，24(4):423-426.(in Chinese)
［22］李小勤，冷向军，吴登俊.国内主要绵羊品种基于染色体核型进化距离的聚类分析［J］.扬州大学学报:农业与生命科学版，2009，30(3):26-30.
LI X Q，LENG X J，WU D J.The cluster analysis of karyotype evolutionary distance in native sheep［J］.Journal of Yangzhou University：Agricultural and Life Science Edition，2009，30(3):26-30.(in Chinese)
［23］BLOMEKE B，GOLKA K，GRIEFAHN B，et al.Arylalkylamine N-acetyltransferase (AA-NAT) genotype as a personal trait in melatonin synthesis ［J］.J Toxicol Environ Health A，2008，71(13-14):874-876.
［24］SANCHEZ-BARCELO E J，MEDIAVILLA M D，ZINN S A，et al.Melatonin suppression of mammary growth in heifers ［J］.Biol Reprod，1991，44(5):875-879.
［25］BORJIGIN J，WANG M M，SNYDER S H.Diurnal variation in mRNA encoding serotonin N-acetyltransferase in pineal gland ［J］.Nature，1995，378(6559):783-785.
［26］FLEMING J V，BARRETT P，COON S L，et al.Ovine arylalkylamine N-acetyltransferase in the pineal and pituitary glands：differences in function and regulation ［J］.Endocrinology，1999，140(2):972-978.
［27］HAMADA T，OOTOMI M，HORIKAWA K，et al.The expression of the melatonin synthesis enzyme：arylalkylamine N-acetyltransferase in the suprachiasmatic nucleus of rat brain ［J］.Biochem Biophys Res Commun，1999，258(3):772-777.
［28］UZ T，MANEV H.Chronic fluoxetine administration increases the serotonin N-acetyltransferase messenger RNA content in rat hippocampus ［J］.Biol Psychiatry，1999，45(2):175-179.
［29］ISORNA E，EI M′RABET A，CONFENTE F，et al.Cloning and expression of arylalkylamine N-acetyltranferase-2 during early development and metamorphosis in the sole Solea senegalensis ［J］.Gen Comp Endocrinol，2009，161(1):97-102.
［30］ISORNA E，ALIAGA-GUERRERO M，M′RABET A E，et al.Identification of two arylalkylamine N-acetyltranferase 1 genes with different developmental expression profiles in the flatfish Solea senegalensis ［J］.J Pineal Res，2011，51(4):434-444.
［31］ISORNA E，BESSEAU L，BOEUF G，et al.Retinal，pineal and diencephalic expression of frog arylalkylamine N-acetyltransferase-1［J］.Mol Cell Endocrinol，2006，252(1-2):11-18.
［32］UZ T，QU T，SUGAYA K，et al.Neuronal expression of arylalkylamine N-acetyltransferase (AA-NAT) mRNA in the rat brain ［J］.Neurosci Res，2002，42(4):309-316.
［33］黄冬维.山羊繁殖季节性调控通路相关基因的克隆、多态与表达研究［D］.北京：中国农业科学院，2012.
HUANG D W.Study on cloning，polymorphisms and expression of genes related with seasonal reproduction pathways in goats ［D］.Beijing：Chinese Academy of Agricultural Sciences，2012.(in Chinese)
［34］杨大前，字向东，王永，等.乐至黑山羊褪黑素合成酶AA-NAT基因cDNA克隆及序列分析［J］.西南民族大学学报:自然科学版，2011，37(4):575-581.
YANG D Q，ZI X D，WANG Y，et al.Molecular cloning and sequence analysis of Lezhi Black-goat melatonin synthetase AA-NAT gene ［J］.Journal of Southwest University for Nationalities：Natural Science Edition，2011，37(4):575-581.(in Chinese)
［35］CHU M X，YAN Y，WANG P Q，et al.Polymorphism of AA-NAT gene and its relationship with litter size of Jining Grey goat of China ［J］.Anim Sci Papers Rep，2013，31(1):15-26.
［36］于成江.常年性和季节性发情绵羊AA-NAT和HIOMT基因遗传多态性的研究［D］.杨凌:西北农林科技大学，2007.
YU C J.Studies on genetic polymorphism of AA-NAT and HIOMT genes in nonseasonal and seasonal estrus sheep［D］.Yangling：Northwest A & F University，2007.(in Chinese)
［37］BAI D P，YU C J，CHEN Y L.Association between AA-NAT gene polymorphism and reproductive performance in sheep ［J］.Elect J Biotechnol，2012，15(2):1-7.
［38］WONGCHITRAT P，GOVITRAPONG P，PHANSUWAN-PUJITO P.The expression of Per1 and AA-NAT genes in the pineal gland of postnatal rats ［J］.J Med Assoc Thai，2012，95(12):S69-75.

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Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep

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